??楂?娓???杩?涓??锛??ㄦ??浠????ュ父??娲讳腑锛???甯镐?瑙?瑙???娓??板害?稿?炽?????ㄥ?哄??绉?瀛﹂???锛???楂?娓????????崇??瀵?NA搴?????缁????????界???寸簿缁????村??纭?????瑙ｃ??????楂?????娴????搴??????NGS锛???椋?????灞?锛???浠??浠ヤ互??????????绮惧害锛?璇诲?????戒????ㄦ???烘?????????锯????
?ㄤ汉绫诲?哄??缁?璁″??????绋?纰?寮???灏变???锛?瀵圭???????哄??缁???楂?娓?娴?搴???娣卞害瑙ｆ??涔??ㄤ????ㄨ??????╁??杩??????╀俊???宸ュ?凤?绉?瀛﹀?浠??藉??????浜虹被?????????????NA楂?娓??捐氨??杩?涓?浠?浠????????纰卞?哄???????锛??存???????????哄????琛ㄨ揪姘村钩??璋??у?哄??????缂???RNA?????斤????虫???缁寸┖?寸?????淇℃????
??浠???互娓??板?扮???帮???涓?涓??哄???ㄤ????╃?涓???绮剧‘浣?缃??瀹?浠???朵??哄?????镐?浣??ㄧ?缁?锛?浠ュ???ㄧ?瑰???????剧???舵??涓???婵?娲绘??娌?榛?妯″???
渚?濡?锛???杩?楂?娓??哄??缁?姣?瀵癸???浠???互绮剧‘?拌????哄?ㄤ汉绫汇????????浣????藉??ㄧ??????婧??哄??????杩?浜??哄??涓?浠??ㄥ???涓?楂?搴︾?镐技锛??撮??瑕??????瀹?浠??ㄥ???戒?涔?寰?寰??????绫讳技??瑙??层??杩?浣垮???浠??藉????村?ㄧ??????韬??宸茬????????哄??娌荤??绛??ワ??ユ??瀵间汉绫荤???剧??娌荤???????凤???涔?浜︾?讹???杩???绌朵汉绫诲???浜??剧?????垫?????????哄?讹???浠???芥?存繁?ュ?扮??瑙ｇ??????????????????
杩?浜??????????斤??界?跺??NA搴??????戒????剁??涓??????╁ぇ?稿?搴??浣??垛??璁捐???蹇碘???村?寰?婧?????版?????藉?哄????娣卞?荤??瑙ｃ??
楂?娓???DNA?捐氨锛?涓?浠?涓烘??浠???渚?浜?浜?瑙ｇ???界?扮?剁??绐?????ｏ??存??涓轰???浠???濉????姐?????????ョ????ヨ???俱???ㄤ汉绫汇???????????哄??浜ゅ???蹭腑锛???浠??㈡????????锛?涔?????插???????绉?????涓????姝ヰ???锛????界?瀛︾??杈圭??灏?涓?????????灞?????浠??????辩?镐俊锛??ㄤ???????灏??ワ??轰?瀵逛汉绫汇????????DNA楂?娓?瑙ｆ????娣卞?ョ??瑙ｏ???浠???藉?浠ユ?存?烘?с???磋?璐ｄ换???瑰?锛?椹鹃┉???界??瀵???锛?涔﹀??灞?浜????ョ????ョ???界?绔???
褰??讹??ㄦ?ユ?辫?浜?婵??ㄤ汉蹇??????芥?ф?讹???浠??椤绘?跺?讳??????璀︽??锛?瀹℃??瀵瑰??哄?????????藉甫?ョ????ヤ鸡????绀句?????锛?纭??绉?????杩?姝ョ??姝ｆ???′??ㄤ汉绫荤??绂?绁???
瀵逛?????瑷?锛??哄??缂?杈??????涓烘?瑰??瀹?浠????ュ悍??绂?绁???渚?浜??伴??寰???渚?濡?锛???互缂?杈??????哄??锛?浣垮?跺???浜???浼??х?剧??锛?濡?????＄????瑙?缃???????绛?锛?浜х???垫????锛?浠???寤堕?垮?浠???瀵垮?斤???楂???娲昏川???????筹???浠???互??杩??哄??缂?杈?锛?杩?涓?姝ヤ?????浜??瑰???绉??????瑰?锛?浠ユ弧瓒充??????浜虹兢????姹?锛?褰??讹?杩??朵腑涔??村????浼︾??涓?????ヨ??????
??褰??哄??缂?杈?????Е??浜虹被??韩?讹?浼︾????杈圭????寰?灏や负??????浠?娌荤????浼??х?剧???板?寮虹?????鸿?斤?浜虹被?哄??缂?杈??????芥?т护浜虹?寰?锛?浣?涔?寮???浜??充???璁捐?濠村?库??浠ュ???哄??楦挎?????蹇с??楂?娓?????ヤ汉绫?NA?捐氨锛?涓烘??浠???渚?浜??寸簿????缂?杈?????锛?浣?濡?浣???瀹???娌荤????涓???澧?寮衡????????锛?濡?浣?纭???哄??缂?杈?????????钩????锛?灏???汉绫荤ぞ浼???瑕??卞???㈠?????澶ц?棰???
???ワ???杩?瀵逛汉绫汇????????DNA楂?娓??捐氨??娣卞?ョ??瑙ｏ???浠???璁歌?藉??存??板?拌?璇??帮?????哄?????瑰?ㄦ????????????锛??????????瀹斥????锛?浠????ㄥ?哄????娴锋?涓???村????板?????
???斤?杩?涓??瀹?涓???浠や汉??杩风???拌薄锛??舵?规?娣卞??浜?寰????DNA涔?涓???褰???浠?话????绌猴????瑰?瀹???娴╃??锛?褰???浠??瑙???韩锛???涓?绂?濂藉????界??璧锋?涓???????浠?澶╋???浠?????︿???浠????????????藉舰寮?????浜虹被锛?浠ュ??涓???浠???娲绘?????稿?崇??涓ょ???瑕????┾??????????????杩?楂?娓???DNA瑙ｆ??锛???浠????ず瀹?浠???撮??缁煎??????哄????绯伙?浠ュ???????ㄧ⒈?哄???涓??????藉?????
涓????哄??????浜茬???涔?璋?锛?浜虹被??????????DNA?镐技?ц??瀵?
涔?涓???锛?浜虹被????????浼间??????朵???????ョ???戒?锛??ユ?????????圭??褰㈡????涔??у????瀛??瑰???浠??哄????灞???搁?㈠?瑙?锛???浠?????颁?涓?护浜烘???圭?????革???浠??变韩???稿?涓??ㄥ??????浼??╄川??杩?绉??镐技?у苟??????剁?讹?????极?挎???杩?绋?涓??卞??绁?????涓????拌???
浜虹被???哄??缁?????ぇ绾?0浜夸釜纰卞?哄?锛???0,000??5,000涓??哄??缂???锛?瀹?浠??卞??orchestrate????浠??????裤?????层??浠ｈ阿浠ュ???????????浣?涓烘??浠???瑕???椋??╂?ユ????诲???绌舵ā??锛??跺?哄??缁?涓?浜虹被??????浜虹???镐技?с????绌惰〃??锛???????ュ?哄??缁??ㄧ????????戒?涓?浜虹被??楂?杈?5%???镐技搴︺??
杩????崇??锛?璁稿??ㄧ??韩涓????????哄??娲诲???涔?涓?浜虹被??????杩?绋?绱у??稿?炽??杩?涓虹???ㄥ?绉绘??颁汉绫昏韩涓?锛?浠ヨВ?冲?ㄥ???己???锛???渚?浜???璁哄?虹???褰??????浠??ㄩ?娓???DNA搴????捐氨涓??瀵癸?浼????颁?浜??抽??????ュ?哄??锛?渚?濡???涓?缁??????裤????????搴????ㄥ????茬???哄??锛??ㄤ汉绫诲????韩涓??介?搴︿?瀹?锛?濡???涓ら?涔??蹭腑??澶??虹?扮??缁??告??寰?锛?浼??????卞???????戒俊????
??浣?涓轰汉绫绘??蹇?璇??????浼翠荆锛??????哄??缁??界?跺?ㄦ?翠?澶у?涓?涓?浜虹被????????涓???锛?浣??朵?浜虹被???哄???镐技搴︿?杈攫????颁???浜虹??85%宸﹀?炽??灏ゅ?舵???ㄤ???瑙????????杩??ㄨ?藉??浠ュ????浜??剧???????х?稿?崇???哄??涓?锛?浜虹被????琛ㄧ?板?烘?捐??????婧??с??渚?濡?锛?涓???瑙???浣??稿?崇???哄??锛??????伴??杩?瓒?浜虹被锛?杩?瑙ｉ??浜?瀹?浠???瓒?????瑙??藉????
??涓?浜?涓?蹇???????????绛??剧???稿?崇???哄??锛??ㄤ汉绫诲??????DNA涓??藉????杩?浣垮???涔???涓虹??绌惰?浜??剧?????ㄤ汉绫昏韩涓??????哄?剁??瀹?璐垫ā????楂?娓????哄??姣?瀵瑰?捐氨锛?濡???缁??跺?轰?骞?骞?绮剧??????撅???ず浜??╃?涔??村?哄??????浜茬????崇郴锛??ｄ???浼奸?ヨ????╃?锛??ㄥ?哄????娣卞?缁村害涓?锛??寸揣瀵??歌???
楂?娓??哄??瑙ｆ??锛?濡???缁????戒?瀹?瑁?浜?涓??扳???惧井????锛?璁╂??浠??藉?瑙?瀵??版??缁?寰????哄??宸??????杩?浜?宸??锛?姝ｆ????翠???????╃?褰㈡???????姐?????宠?涓哄樊寮????规????????渚?濡?锛??у?舵???棰??层??浣???澶у??????崇????规????琛?涓烘ā寮?锛??藉??互杩芥函??NA涓?井灏???纰卞?虹??????哄???疯??扮????????
瀵逛?????瑷?锛?璇稿????块??搴︺??浜ц???藉??绛?缁?娴??х?讹?涔?涓??瑰????哄????寮??????稿?炽??楂?娓????哄???捐氨锛?涓烘??浠???ず杩?浜????藉ゥ绉?锛???渚?浜???????????娓??拌?瑙???瀹?涓?浠?浠????瀛︾??绌剁????ュ伐?凤??存????ヤ??????藉舰寮?锛???瑙ｇ???芥??川???虹?炽??
part1??浠?凡缁?娣卞?ユ?㈢储浜?浜虹被????????DNA楂?娓??捐氨??濂ョ?锛????颁?瀹?浠???存繁?荤????浜茬?????绯伙?浠ュ??楂?娓??哄??瑙ｆ???????ㄦ??ず???芥??川?归?㈢??寮哄ぇ浣??ㄣ???板???璁╂??浠?????????????ワ????冲?轰?杩?浜??哄??璁ょ?ワ????藉??插?虹?????????姐??
涓????哄??缂?杈?涓???瀹??垛?????斤?????????浜虹被??????浣????
CRISPR-Cas9绛??哄??缂?杈????????虹?帮??????????界?瀛﹂?????涓??洪?╁?姐??杩?椤规?????浜?浜???浠????????????藉??锛??藉?绮剧‘?颁慨??NA搴???锛?瀹??板?哄????????????涓???绮?璐粹????褰???浠??杩?椤规??????ㄤ?浜虹被??????????DNA?讹?涓?绯诲??浠や汉?村?????婊′?璁??????芥?т究搴?杩???????
?ㄥ?昏?????锛?浠ョ??负妯″?????哄??缂?杈???绌跺凡??寰??捐??杩?灞?????杩?缂?杈???????ュ?哄??锛???互娑??ゅ?朵??????藉?璧蜂汉绫诲???????ョ???哄??锛?????寮??ヤ汉绫诲?哄??浠ヤ娇?跺?ㄥ??存??琚?汉绫绘?ュ????杩?涓哄?绉??ㄥ?绉绘?锛?瑙ｅ?冲?ㄧ???ㄥ???己??涓ュ郴???锛???寮?浜?涓????扮??澶ч?ㄣ???宠薄涓?涓?锛???杩?楂?娓????哄???捐氨锛???浠???互绮剧‘璇???苟淇?????哄??缁?涓????藉?璧蜂汉绫绘???ョ??????纰???锛?璁╃????涓哄?????跺?????浜虹被?ㄥ?澶?浠跺?????
?や??哄??缂?杈?锛????????╁??存?????浠???哄???????ф?ㄥ??浜??扮??楂?搴︺??瀹??ㄥ?ㄩ??杩?宸ョ??????规?锛?璁捐?????寤烘?扮?????╅?ㄤ欢??璁惧???绯荤?锛????冲?????鸿???剁??涓?瀛??ㄧ?????藉舰寮?????楂?娓???DNA淇℃???姝ｆ?????????╁?寰?浠ユ?藉?????涔?楂?绉??ㄢ????
璁炬?充?涓?锛?濡?????浠??藉?????璇烩??浜虹被????????DNA????楂?娓?璇存??涔︹??锛?骞朵????涓?????????浠峰?肩???????芥ā????锛??跺??灏?杩?浜?妯″?????扮???锛????冲?????吼??″?ㄦ?扮???哄??搴???锛?杩?灏?浼?甯︽?ユ???风??棰?瑕?锛?渚?濡?锛???浠???互?╃?ㄧ??????浜??哄???规?э??モ??璁捐????哄?锋???规???娉????界??缁???锛??ㄤ???浜ц???╂?????╂??????
????锛???浠???互???寸??????浜??哄??锛??モ??缂?绋????鸿?藉?绮惧??璇?????娓??ょ??缁???????瓒?绾у???????????
???????╁???缁???????涔?涓?锛????????哄?锋???ㄦ?板???界???????????解????灏界?璺?绂诲??ㄤ??跺?濮?????涓?涓???????戒?杩???寰??跨??璺??璧帮?浣???杩?瀵逛汉绫汇????????绛????澶??????╁?哄??缁???楂?娓?瑙ｆ??锛???浠?凡缁?绉?疮浜?涓板?????璁捐?绱???????渚?濡?锛?绉?瀛﹀?浠???ㄥ?璇??????锋???瑰????界??缁???锛??ㄤ????╀慨澶????芥???浜х?棰?????
涓?ぎ缃?淇″??杩?娉???涓???俊??妇?ヤ腑蹇?|璇??告?舵?ョ?涓炬?ヤ腑蹇?
?靛???/a>
